Department of Translational Medicine, Italy

Dr. Marcello Manfredi
Dr. Marcello Manfredi

Department of Translational Medicine, Italy

Hospitals involved

Novara Hospital, Azienda Ospedaliero Universitaria Maggiore della Carità di Novara

Type of analysis

1) Untargeted metabolomics by GCxGC-MS following derivatization. 2) Untargeted lipidomics by LC-HRMS

Outputs

Protocol used

1) Protein precipitation with ACN/IPA/MeOH, dry in speed-vac, followed by MeOx and BSTFA derivatization, 2. lipid extraction with MeOH – MTBE – H2O (Cajka, Metabolomics 12:127, 2016)