Hospitals involved
Novara Hospital, Azienda Ospedaliero Universitaria Maggiore della Carità di Novara
Type of analysis
1) Untargeted metabolomics by GCxGC-MS following derivatization. 2) Untargeted lipidomics by LC-HRMS
Outputs
Protocol used
1) Protein precipitation with ACN/IPA/MeOH, dry in speed-vac, followed by MeOx and BSTFA derivatization, 2. lipid extraction with MeOH – MTBE – H2O (Cajka, Metabolomics 12:127, 2016)